[Cnqo] Fwd: [Physstaff] Colloquium: Paola Borri (Cardiff) Coheren Multi-Photon Microscopy 3pm Wed 19/3/2014

[Gian-Luca Oppo]

Di sicuro.

Puoi chiedere anche a Gail McConnell (g.mcconnell at strath.ac.uk<mailto:g.mcconnell at strath.ac.uk>) che lavora su queste cose?
Grazie.

CIao,

gian-luca

--
Gian-Luca Oppo
g.l.oppo at strath.ac.uk<mailto:g.l.oppo at strath.ac.uk>



On 17 Mar 2014, at 21:16, Francesco Papoff <f.papoff at strath.ac.uk<mailto:f.papoff at strath.ac.uk>> wrote:

Dear All,

Prof Paola Borri will be around most of Wednesday, please let me know if you wish to talk to her.

Best regards

Francesco

________________________________________________________________________________________________________________

We are happy to have Prof Paola Borri (Cardiff) give this week’s colloquium. As usual, there will be tea/coffee afterwards.

Title: Shedding new light on cells with coherent multiphoton microscopy
Time/Date: 3pm Wednesday 19th March 2014
Room: JA8.07

Abstract:
Optical microscopy is an indispensable tool that is driving progress in cell biology, and is still the only practical means of obtaining spatial and temporal resolution within living cells and tissues. Much effort is being devoted recently to achieve intrinsic three-dimensional (3D) spatial resolution by exploiting optical nonlinear effects which can only take place in the small focal volume where high photon densities are reached. One of the most utilised multiphoton (ie nonlinear) microscopy techniques is two-photon fluorescence where the biomolecules of interest are labelled with fluorophores which are optically excited via simultaneous absorption of two photons. However, these modified biomolecules raise questions if their behaviour is real or artefactual. Furthermore, all organic fluorophores are prone to photo-bleaching which severely limits time-course observations and is accompanied by toxicity effects and consequent cell damage.

Coherent Antistokes Raman Scattering (CARS) microscopy has recently emerged as a new multiphoton microscopy technique which overcomes the need of fluorescent labelling and yet retains biomolecular specificity and intrinsic 3D resolution [1]. We have developed in our laboratory two home-built CARS microscopes featuring innovative CARS excitation/detection schemes. In particular, we have demonstrated dual frequency differential-CARS (DCARS) with strongly suppressed non-resonant background and improved chemical sensitivity [2] and single-laser CARS [3] utilising femtosecond laser pulses linearly chirped by glass dispersion. Our second generation single-broadband-laser D-CARS microscope features a single 5fs Ti:Sa source with 350nm bandwidth capable of exciting a wide vibrational range from 1000cm-1 to 3500cm-1 [4] thus enabling powerful hyperspectral microscopy analysis [5]. Furthermore we have invented and demonstrated a novel imaging modality, based on the resonant Four-Wave Mixing (FWM) of colloidal nanoparticles. Results on this work showed that gold nanoparticles can be used as alternative labels beyond fluorescence by exploiting their resonant FWM, to achieve a novel coherent multiphoton microscopy modality free from background and with a spatial resolution significantly surpassing the one-photon diffraction limit [6]. I will present our latest progress with both techniques and their applications to cell imaging.

References
[1] Andreas Zumbusch, Wolfgang Langbein, Paola Borri “Nonlinear vibrational microscopy applied to lipid biology” Progress in Lipid Research 52, 615 (2013).
[2]  Israel Rocha-Mendoza  et al “Differential Coherent Antistokes Raman scattering Microscopy using linearly-chirped ultrafast laser pulses”, Optics Letters 34, 2258 (2009), ibid “Quadruplex CARS micro-spectroscopy”, J. Raman Spectrosc. 44, 255 (2013).
[3] Wolfgang Langbein et al “Single source coherent anti-Stokes Raman micro-spectroscopy using spectral focusing”, Applied Physics Letters 95, 081109 (2009).
[4] Claudia Di Napoli et al “Chemically-specific dual/differential CARS micro-spectroscopy of saturated and unsaturated lipid droplets”, Journal of Biophotonics 7, 68 (2014); I. Pope et al “Simultaneous hyperspectral differential-CARS, TPF and SHG microscopy with a single 5 fs Ti:Sa laser” Optics Express 21, 7096 (2013).
[5] Francesco Masia et al “Quantitative chemical imaging and unsupervised analysis using hyperspectral coherent anti-Stokes Raman scattering microscopy” Anal. Chem. 85, 10820 (2013).
[6] Francesco Masia et al “Resonant four-wave mixing of gold nanoparticles for three-dimensional cell microscopy”, Optics Letters 34, 1816 (2009). ibid “Measurement of the dynamics of plasmons inside individual gold nanoparticles using a femtosecond phase-resolved microscope” Phys. Rev B 85 235403 (2012).

http://www.strath.ac.uk/physics/research/colloquia/
--------
Dr Daniel K. L. Oi
Lecturer in Quantum Information
Computational Non-Linear & Quantum Optics
SUPA Department of Physics
University of Strathclyde
Glasgow G4 0NG, UK

Tel: +44 141 548 3112
Fax: +44 141 552 2891

The University of Strathclyde is a charitable body, registered in Scotland, number SCO15263


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