[Postgrad] FW: Seminar, 9th November

[Neil Hunt]

Dear All,



Please see below details of a seminar which may be of interest.



Best wishes,

Neil





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A seminar will be given by Dr Brian Patton, Royal Society University Research Fellow, University of Oxford, and will take place on Monday 9th November at 1pm in JA5.07. Please see below for the title and abstract of his talk. All welcome.



Super-resolution, synapses and sparkle!

Brian Patton

Centre for Neural Circuits and Behaviour, Oxford University, Mansfield Road, Oxford, OX1 3SR



The development of super-resolution microscopy, in which structures significantly smaller than the wavelength of light are imaged, has encompassed both scanning methods (STED, RESOLFT, etc.) and stochastic wide field methods (PALM, STORM, GSDIM, etc.). The demanding nature of the imaging formation process means that aberrations induced by the sample can significantly compromise resolution and signal beyond that seen in conventional, diffraction-limited methodologies. We have been investigating the incorporation of adaptive optics technologies into super-resolution microscopes in order to correct for these aberrations and allow effective imaging in otherwise unsuitable samples. In particular, I will demonstrate how, by combining a deformable mirror with a spatial light modulator in a STED microscope, we can image fruit fly (Drosophila Melanogaster) brains at depths where a conventional STED microscope would fail to return any images.



I have also been investigating the use of nanodiamond as a fluorophore. Its size, biocompatibility and ability to be functionalised make it suitable for a wide range of imaging modalities and sample types. In particular, defects such as the nitrogen vacancy are sensitive to electric and magnetic fields, opening the possibility of in-vivo measurement of biologically generated fields at the cellular scale. In order to implement nanodiamond biosensing it is important to target the nanoparticles to structures of interest, image them efficiently and perform quantum optical measurements to correlate emission with the local electromagnetic environment. I will present here the current status of my research programme which aims to utilise the NDs to image neural structures and activity in Drosophila brains.




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