[Physstaff] Fwd: Seminar: Professor Fu-Jen Kao, 4th August

[David McKee]

Interesting seminar taking place in the JA building next week. See below.

Dear all,

Professor Fu-Jen Kao from National Yang-Ming University, Taipei, will 
give a seminar on Thursday 4th August. The seminar will take place in 
JA5.06 from 12noon-1pm. Details below, and all are welcome.

*Stimulated **E**mission **B**ased **F**luorescence **D**etection*

*I-Che Chen, Chun-Hui Yu**, and Fu-Jen Kao**

/Institute of Biopho//to//nics, National Yang-Ming University, //Taipei 
11221, Taiwan, ROC///**

Fluorescence is widely used in biomedical imaging. It allows specific 
labelingand targetingwithmolecular sensitivitythrough 
immunocytochemistry.However, itis incoherent and emits in all 
directions.Henceforthits intensity decreases rapidly and inversely with 
the square of the distanceandwould require high numerical aperture (NA) 
opticsfor efficientcollection. This problem can be remediedby stimulated 
emission (SE) based fluorescence detection that would maintain the 
molecule specific contrast through immuno-labeling whileconvertingthe 
incoherent spontaneous fluorescence signal into a stimulated and 
coherent one. We had demonstrated stimulated emission (SE) imaging ina 
long-working distance configuration. Additionally, the fluorescence 
lifetime imaging is realized by electronically controlling thetime delay 
between the excitation and the SE pulses in the nanosecond rangesthrough 
pump-probe configuration.However, the depth resolution is sacrificed due 
tolow NA optics in such a configuration. In order to take advantage 
ofthe optical coherence of SE and obtain thecorresponding depth 
information,interferometrictechniques areincorporated.We have obtained a 
resolution of 66 mm, which depends on the coherence length of the 
stimulation beam.


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Professor Gail McConnell
Centre for Biophotonics
SIPBS Building, University of Strathclyde
161 Cathedral Street
Glasgow G4 0RE
Tel: 00 44 141 548 4805
Email: g.mcconnell at strath.ac.uk
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