[Physstaff] FW: Seminar: Professor Fu-Jen Kao, 4th August

[Audrey McKinnon]

SENT ON BEHALF OF PROF. GAIL  MCCONNELL


Dear all,

Professor Fu-Jen Kao from National Yang-Ming University, Taipei, will give a seminar on Thursday 4th August. The seminar will take place in JA5.06 from 12noon-1pm. Details below, and all are welcome.


Stimulated Emission Based Fluorescence Detection

I-Che Chen, Chun-Hui Yu, and Fu-Jen Kao*
Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan, ROC

Fluorescence is widely used in biomedical imaging. It allows specific labeling and targeting with molecular sensitivity through immunocytochemistry. However, it is incoherent and emits in all directions. Henceforthits intensity decreases rapidly and inversely with the square of the distance and would require high numerical aperture (NA) optics for efficient collection. This problem can be remedied by stimulated emission (SE) based fluorescence detection that would maintain the molecule specific contrast throughimmuno-labeling while converting the incoherent spontaneous fluorescence signal into a stimulated andcoherent one. We had demonstrated stimulated emission (SE) imaging in a long-working distanceconfiguration. Additionally, the fluorescence lifetime imaging is realized by electronically controlling thetime delay between the excitation and the SE pulses in the nanosecond ranges through pump-probe configuration. However, the depth resolution is sacrificed due to low NA optics in such a configuration. In order to take advantage of the optical coherence of SE and obtain the corresponding depth information,interferometric techniques are incorporated. We have obtained a resolution of 66 mm, which depends on the coherence length of the stimulation beam.


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Professor Gail McConnell
Centre for Biophotonics
SIPBS Building, University of Strathclyde
161 Cathedral Street
Glasgow G4 0RE

Tel: 00 44 141 548 4805
Email: g.mcconnell at strath.ac.uk<mailto:g.mcconnell at strath.ac.uk>
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